Fig. 4

Activation of LTR-TEs in K2 male germline and the surrounding DNA methylation. A Heatmap showing the expression level of active LTR-TEs, which can be divided into four clusters, in leaf, meiocyte, microspore and pollen of K2 (left panel). Scale bar represents the z-score of the normalized expression values. Three biologic replicates were performed for RNA-seq with sex cells or leaf tissue (see Methods). B Curved line graph displaying the average CG/CHG/CHH methylation ratio around the Cluster1 and inactive LTR-TEs in leaf, meiocyte, microspore and pollen. Two biologic replicates were performed for WGBS with sex cells or leaf tissue in K2 (see Methods). Mc, meiocyte; Msp, microspore; Pln, pollen. C The bar charts showing the percentage of each family in active, Cluster 1, Cluster 2, Cluster 3, Cluster 4, inactive and all LTR-TEs. D The bar charts showing the percentage of fragment length for active and inactive LTR-TEs