Fig. 3
From: DNA methylation dynamics in male germline development in Brassica Rapa
Expression pattern of key genes in DNA methylation and demethylation and DNA methylation at RdDM target loci in K2 male germline. A Heatmap showing transcriptome levels of key component genes of the maintenance pathways, RdDM pathway and demethylation pathway in leaf, meiocyte, microspore and pollen of K2. Mc, meiocyte; Msp, microspore; Pln, pollen. Scale bar represents the z-score of the normalized expression values. Three biologic replicates were performed for RNA-seq with sex cells or leaf tissue (see Methods). B Box plots illustrating the absolute methylation at 1000 randomly selected loci (mark the ‘Random loci’ on X-axis) in R-o-18[25] (left panel) and their homologous sites in K2 (right panel), leaf RdDM target loci of R-o-18[25] (mark ‘leaf RdDM loci’ on X-axis) (left panel) and their homologous sites in K2 (right panel), ovule RdDM target loci in ovule of R-o-18[25] (mark ‘Ovule RdDM loci’ on X-axis) (left panel) and their homologous sites in K2 (right panel), Siren loci of R-o-18[25] (mark ‘Siren loci’ on X-axis) (left panel) and their homologous sites in K2 (right panel). Two biologic replicates were performed for WGBS with sex cells or leaf tissue in K2 (see Methods), three biologic replicates were analyzed for WGBS with ovule, endosperm or leaf tissue in R-o-18[25], and two biologic replicates were analyzed for WGBS with early or mature embryo in R-o-18[25]. Box plots were generated on the basis of 50-bp windows with substantial methylation in all cell types in the corresponding sequence context with the horizontal line marks the median