Fig. 1

Global DNA methylation of transposons and genes in K2. A K2 transposons (left) or genes (right) were aligned at the 5’ and 3’ ends (dashed lines) with average methylation in the CG, CHG or CHH context for each 100-bp interval were plotted for leaf, meiocyte, microspore and pollen. Two biologic replicates were performed for WGBS with leaf or sex cells in K2 (see Methods) CG methylation is shown in the first row, CHG in the middle row, CHH in the last row (B) Box plots showing the absolute methylation in K2 leaf, meiocyte, microspore and pollen, and R-o-18 leaf, ovule, early embryo, mature embryo and endosperm at SLM-like loci and canonical SLH-like loci (see text). Two biologic replicates were performed for WGBS with sex cells or leaf tissue in K2 (see Methods), three biologic replicates were analyzed for WGBS with ovule, endosperm or leaf tissue in R-o-18^[25], and two biologic replicates were analyzed for WGBS with early or mature embryo in R-o-18^[25]. Box plots were generated on the basis of 50-bp windows with substantial methylation in all cell types in the corresponding sequence context with the horizontal line marks the median